Guest posting: Dr Ian Marr, Microbiology Registrar, NSW Health Pathology.
Malaria diagnosis Marr August 2018 v2
Short questions for postgraduates:
- Describe the relative sensitivity of different malaria test methodologies – microscopy, rapid diagnostic kits and nucleic acid amplification.
- Describe the process of malarial slide preparation and staining emphasising the critical factors
- Explain how one examines malarial microscopy slides
- What is the relevance of microscopy/RDT negative malaria cases?
- How are RDTs evaluated by WHO? How well do the RDTs in use in PNG perform according to the most recent WHO report?
- What are critical factors that affect the sensitivity of RDTs?
- What is the LAMP method and how is the test conducted? (Consider preanalytical, analytical and post-analytical steps)
Basic malaria microscopy – Part I: Learner’s guide. Second edition, February 2010 – World Health Organisation
- Imwong et al. The epidemiology of subclinical malaria infections in South‑East Asia: findings from cross‑sectional surveys in Thailand–Myanmar border areas, Cambodia, and Vietnam. Malar J (2015) 14:381. – the important role of PCR in surveillance and disease control.
- Mathison et al. Update on Malaria Diagnostics. JCM 2017 – excellent short review.
- Lab capacity PNG_Evelyn Lavu_Global Malaria Congress 2018– important recent overview of malaria situation in PNG and laboratory capacity
- Rosewell-A et al. Health information system strengthening and malaria elimination
in Papua New Guinea. Malar J (2017) 16:278.