Author Archives: mdjkf

About mdjkf

Microbiologist and Infectious Diseases Physician

Bacteriology 101

Pre-analytical issues Correct indication for testing – concept of ‘diagnostic stewardship’ Patient identification and sample labelling Correct specimen collection- avoid contamination- esp. blood culture Correct transport and storage Reject poor samples – sputum with no PMNs squames++, urinary catheters, wound … Continue reading

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Enzyme immunoassays (EIA) unpacked

There are many types of EIA used in microbiology and biochemistry.  Depending on the format of the test, some have alternative names such as ELISA and CLIA (chemoluminescent immunoassay).

Posted in Module-virology, Part 2 M Med Micro topics | Tagged | Leave a comment

Laboratory diagnosis of fungal disease- selective overview

Organism Microscopy Culture and identification Non-culture methods General Histopath PAS & silver stains etc. K0H with Parker ink (skin scrapings/nails) SAB (Saborauds dextrose agar) medium +/- antibiotics +/- cycloheximide For other media, see here. Blue thymol blue sticky tape preparation … Continue reading

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Tuberculosis update 2018 from A/Prof Anna Ralph

Guest posting: Associate Professor Anna Ralph, Director of Global and Tropical Health at Menzies School of Health Research, Darwin, Northern Territory, Australia.  News :  UN General Assembly first ever High-Level Meeting on the fight against tuberculosis, September 28th 2018. New(ish) WHO … Continue reading

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Video masterclass- part 3- Faeces plate reading- Dr Chris Ashhurst-Smith

Guest posting: Dr Chris Ashhurst-Smith, Pathology North. Part 3: Faeces culture plate reading.  Video Felix Ferguson.

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Video masterclass – Dr Chris Ashhurst-Smith – part 2- urine culture reading

Guest posting: Dr Chris Ashhurst-Smith, Pathology North. Here is part 2 about urine culture reading. Video by Felix Ferguson. Thank you Chris! Enjoy.

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Practical bacteriology bench reading – video masterclasses from Dr Chris Ashhurst-Smith

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Malaria diagnostics- what’s new and why is it important?

Guest posting: Dr Ian Marr, Microbiology Registrar, NSW Health Pathology.  Malaria diagnosis Marr August 2018 v2 Short questions for postgraduates: Describe the relative sensitivity of different malaria test methodologies – microscopy,  rapid diagnostic kits and nucleic acid amplification. Describe the … Continue reading

Posted in Module - Parasitology, Protozoa | Tagged | Leave a comment

Genetics presentations L/Professor Rodney Scott, Bir Hospital, Nepal August 2018

Guest posting with permission from Laureate Professor Rodney Scott : these are his presentations provided to post-graduates in Nepal during his recent visit to inspect the new cytogenetics laboratory at the National Academy of Medical Sciences,  Bir Hospital, Kathmandu. Most presentations … Continue reading

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Parasitology overview and helminth infection (PNG)

Classification of Parasites and Helminths 2018 Marr Diagnosis of filarial infections (WHO) Kow PNGMJ 2013 – a comparative study of intestinal helminths, PNG Owen-IL Parasitic_zoonoses_PNG J Helminthology 2005 Strongyloides CDC site ;   Robertson NT strongyloides PCR test Reference CDC  Laboratory Identification … Continue reading

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