Author Archives: mdjkf

About mdjkf

Microbiologist and Infectious Diseases Physician

Entamoeba histolytica

Case report 1- SIDG 2017_Dr C Swan Amoebiasis PNG case report:  Multiple liver abscesses: an unusual case which demonstrates the importance of ultrasonography in the detection of liver pathology. Life cycle Rapid diagnostic tests are reliable now – distinguish E. histolytica … Continue reading

Posted in Module - Parasitology, Protozoa | Tagged | Leave a comment

Antimicrobial susceptibility testing – video masterclasses – Fiona Courtney

Consumables for AST – Set up of AST – Reading of the AST result – Thank you Fiona for these excellent explanations!  

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How to do a minor research thesis (for instance an M. Med study report)

Here is an excellent overview from Professor Trevor Duke who is a conjoint academic with UPNG.  Follow his advice when setting up the study and writing its report. Abstract:  Many universities and colleges in low-income and middle-income countries require a masters … Continue reading

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Bacteriology 101

Pre-analytical issues Correct indication for testing – concept of ‘diagnostic stewardship’ Patient identification and sample labelling Correct specimen collection- avoid contamination- esp. blood culture Correct transport and storage Reject poor samples – sputum with no PMNs squames++, urinary catheters, wound … Continue reading

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Enzyme immunoassays (EIA) unpacked

There are many types of EIA used in microbiology and biochemistry.  Depending on the format of the test, some have alternative names such as ELISA and CLIA (chemoluminescent immunoassay).

Posted in Med Micro topics-advanced, Module-virology | Tagged | Leave a comment

Laboratory diagnosis of fungal disease- selective overview

Organism Microscopy Culture and identification Non-culture methods General Histopath PAS & silver stains etc. K0H with Parker ink (skin scrapings/nails) SAB (Saborauds dextrose agar) medium +/- antibiotics +/- cycloheximide For other media, see here. Blue thymol blue sticky tape preparation … Continue reading

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Tuberculosis update 2018 from A/Prof Anna Ralph

Guest posting: Associate Professor Anna Ralph, Director of Global and Tropical Health at Menzies School of Health Research, Darwin, Northern Territory, Australia.  News :  UN General Assembly first ever High-Level Meeting on the fight against tuberculosis, September 28th 2018. New(ish) WHO … Continue reading

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Video masterclass- part 3- Faeces plate reading- Dr Chris Ashhurst-Smith

Guest posting: Dr Chris Ashhurst-Smith, Pathology North. Part 3: Faeces culture plate reading.  Video Felix Ferguson.

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Video masterclass – Dr Chris Ashhurst-Smith – part 2- urine culture reading

Guest posting: Dr Chris Ashhurst-Smith, Pathology North. Here is part 2 about urine culture reading. Video by Felix Ferguson. Thank you Chris! Enjoy.

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Practical bacteriology bench reading – wound swabs and respiratory plates – video masterclasses from Dr Chris Ashhurst-Smith

Posted in Module-Respiratory infections, Module-Skin soft tissue infection | Tagged , | Leave a comment