Category Archives: Microbiology

Entamoeba histolytica

Case report 1- SIDG 2017_Dr C Swan Amoebiasis PNG case report:  Multiple liver abscesses: an unusual case which demonstrates the importance of ultrasonography in the detection of liver pathology. Life cycle Rapid diagnostic tests are reliable now – distinguish E. histolytica … Continue reading

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Antimicrobial susceptibility testing – video masterclasses – Fiona Courtney

Consumables for AST – Set up of AST – Reading of the AST result – Thank you Fiona for these excellent explanations!  

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Laboratory diagnosis of fungal disease- selective overview

Organism Microscopy Culture and identification Non-culture methods General Histopath PAS & silver stains etc. K0H with Parker ink (skin scrapings/nails) SAB (Saborauds dextrose agar) medium +/- antibiotics +/- cycloheximide For other media, see here. Blue thymol blue sticky tape preparation … Continue reading

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Tuberculosis update 2018 from A/Prof Anna Ralph

Guest posting: Associate Professor Anna Ralph, Director of Global and Tropical Health at Menzies School of Health Research, Darwin, Northern Territory, Australia.  News :  UN General Assembly first ever High-Level Meeting on the fight against tuberculosis, September 28th 2018. New(ish) WHO … Continue reading

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Malaria diagnostics- what’s new and why is it important?

Guest posting: Dr Ian Marr, Microbiology Registrar, NSW Health Pathology.  Malaria diagnosis Marr August 2018 v2 Short questions for postgraduates:

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Parasitology overview and helminth infection (PNG)

Classification of Parasites and Helminths 2018 Marr Diagnosis of filarial infections (WHO) Kow PNGMJ 2013 – a comparative study of intestinal helminths, PNG Owen-IL Parasitic_zoonoses_PNG J Helminthology 2005 Strongyloides CDC site ;   Robertson NT strongyloides PCR test Reference CDC  Laboratory Identification … Continue reading

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Burkholderia pseudomallei review including laboratory aspects

Guest posting: Dr Ian Marr, Microbiology Registrar, Pathology North. Melioidosis is a cause of severe pneumonia, bacteraemia, urosepsis, prostatic abscess, CNS infection, pyogenic myositis and liver and spleen abscesses. It is found in PNG especially around the Balimo region – … Continue reading

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Virology overview

Consider specific diagnostic approaches to each agent, including where relevant, serological testing, antigen detection and nucleic acid amplification methods.  Also know what is locally in use in your laboratory. Immunisation – need to know about the vaccines that are available … Continue reading

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How to read the cumulative antibiogram and its usefulness

Here are example antibiograms for Hunter New England (NSW). These are constructed by assessing the % susceptible for specific bugs and drugs, taking only the first isolate of a species per person per annum.  Where is it possible extrapolate susceptibility … Continue reading

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Nocardia and aerobic actinomycetes

Guest posting: Dr Syeda Navqi, Microbiology Registrar, Pathology North.  Group: Aerobic actinomycetes Gram positive bacteria that are usually filamentous and branched, commonly producing a fungus like mycelium that fragments into rod shaped or short coccoid form. All grow better under … Continue reading

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