Author Archives: mdjkf

Unknown's avatar

About mdjkf

Microbiologist and Infectious Diseases Physician

Entamoeba histolytica

Posted on February 28, 2019 by mdjkf

Case report 1- SIDG 2017_Dr C Swan Amoebiasis PNG case report:  Multiple liver abscesses: an unusual case which demonstrates the importance of ultrasonography in the detection of liver pathology. Life cycle Rapid diagnostic tests are reliable now – distinguish E. histolytica … Continue reading →

Posted in Module - Parasitology, Protozoa | Tagged | Leave a comment

Antimicrobial susceptibility testing – video masterclasses – Fiona Courtney

Posted on February 6, 2019 by mdjkf

Consumables for AST – Set up of AST – Reading of the AST result – Thank you Fiona for these excellent explanations!  

Posted in Antimicrobial susceptibility testing, Module-Skin soft tissue infection | Tagged , | Leave a comment

How to do a minor research thesis (for instance an M. Med study report)

Posted on February 6, 2019 by mdjkf

Here is an excellent overview from Professor Trevor Duke who is a conjoint academic with UPNG.  Follow his advice when setting up the study and writing its report. Abstract:  Many universities and colleges in low-income and middle-income countries require a masters … Continue reading →

Posted in Research | Tagged | Leave a comment

Bacteriology 101

Posted on September 23, 2018 by mdjkf

Pre-analytical issues Correct indication for testing – concept of ‘diagnostic stewardship’ Patient identification and sample labelling Correct specimen collection- avoid contamination- esp. blood culture Correct transport and storage Reject poor samples – sputum with no PMNs squames++, urinary catheters, wound … Continue reading →

Posted in Module-basic microbiology and AMR | Tagged | Leave a comment

Enzyme immunoassays (EIA) unpacked

Posted on September 22, 2018 by mdjkf

There are many types of EIA used in microbiology and biochemistry.  Depending on the format of the test, some have alternative names such as ELISA and CLIA (chemoluminescent immunoassay).

Posted in Med Micro topics-advanced, Module-virology | Tagged | Leave a comment

Laboratory diagnosis of fungal disease- selective overview

Posted on September 21, 2018 by mdjkf

Organism Microscopy Culture and identification Non-culture methods General Histopath PAS & silver stains etc. K0H with Parker ink (skin scrapings/nails) SAB (Saborauds dextrose agar) medium +/- antibiotics +/- cycloheximide For other media, see here. Blue thymol blue sticky tape preparation … Continue reading →

Posted in Fungi, Module-Mycology | Leave a comment

Tuberculosis update 2018 from A/Prof Anna Ralph

Posted on September 19, 2018 by mdjkf

Guest posting: Associate Professor Anna Ralph, Director of Global and Tropical Health at Menzies School of Health Research, Darwin, Northern Territory, Australia.  News :  UN General Assembly first ever High-Level Meeting on the fight against tuberculosis, September 28th 2018. New(ish) WHO … Continue reading →

Posted in Module- Mycobacteria, Mycobacteria tuberculosis | Leave a comment

Video masterclass- part 3- Faeces plate reading- Dr Chris Ashhurst-Smith

Posted on September 18, 2018 by mdjkf

Guest posting: Dr Chris Ashhurst-Smith, Pathology North. Part 3: Faeces culture plate reading.  Video Felix Ferguson. 8 minutes.

Posted in Module-GIT and intra-abdominal | Tagged , | Leave a comment

Video masterclass – Dr Chris Ashhurst-Smith – part 2- urine culture reading

Posted on September 17, 2018 by mdjkf

Guest posting: Dr Chris Ashhurst-Smith, Pathology North. Here is part 2 about urine culture reading. Video by Felix Ferguson. Thank you Chris! Enjoy.

Posted in Module-Urinary tract infection | Tagged , | Leave a comment

Practical bacteriology bench reading – wound swabs – video masterclasses from Dr Chris Ashhurst-Smith

Posted on September 16, 2018 by mdjkf

  20 minutes. Filming and production Felix Ferguson.

Posted in Module-Skin soft tissue infection | Tagged , | Leave a comment