Video masterclass – Dr Chris Ashhurst-Smith – part 2- urine culture reading

Guest posting: Dr Chris Ashhurst-Smith, Pathology North.

Here is part 2 about urine culture reading. Video by Felix Ferguson. Thank you Chris! Enjoy.

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Practical bacteriology bench reading – wound swabs and respiratory plates – video masterclasses from Dr Chris Ashhurst-Smith

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Malaria diagnostics- what’s new and why is it important?

Guest posting: Dr Ian Marr, Microbiology Registrar, NSW Health Pathology. 

Malaria diagnosis Marr August 2018 v2

Short questions for postgraduates:

Continue reading

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Genetics presentations L/Professor Rodney Scott, Bir Hospital, Nepal August 2018

Guest posting with permission from Laureate Professor Rodney Scott : these are his presentations provided to post-graduates in Nepal during his recent visit to inspect the new cytogenetics laboratory at the National Academy of Medical Sciences,  Bir Hospital, Kathmandu. Most presentations have annotations. 

BRCA1 and BRCA2 Structure:

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Parasitology overview and helminth infection (PNG)

Reference

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Tuberculosis – recent epidemiological data from PNG

These recent papers will be useful to review/ discuss (especially the first two):

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Measles – always relevant

Guest posting: Dr Nilar Lwin, Microbiology Registrar, Pathology North. 

Measles overview including laboratory diagnosis:    Measles virus Lwin 2018

Reports of recent measles outbreaks in PNG which are worth a close read:

References

Measles rash, day 3                                                  Koplik spots

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Immunisation schedules and SAQ

It’s useful to know your schedules and appreciate something of the rationale for the dosing recommendations. Also be aware of key vaccines that are yet to be introduced – e.g. rotavirus, HPV.  Please work through the SAQ below.  Correct 2016 version of Immunisation schedule obtained from PNG Paediatric site, 2018.

PNG IMM 2016

In the news:

Some short questions to consider:

  1. Contrast the PNG and NHS schedules above.  What important gaps are there?
  2. Why has IPV replaced OPV in the NHS schedule?
  3. What is happening with wild type polio control across the world? Which subtype has been eradicated? Where are the remaining areas of activity for wild type polio?
  4. How does OPV revert to virulence?
  5. What are the risks of dengue vaccine? What is its efficacy in different groups?
  6. How does one estimate vaccine efficacy?

Reference

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Burkholderia pseudomallei review including laboratory aspects

Guest posting: Dr Ian Marr, Microbiology Registrar, Pathology North.

Melioidosis is a cause of severe pneumonia, bacteraemia, urosepsis, prostatic abscess, CNS infection, pyogenic myositis and liver and spleen abscesses. It is found in PNG especially around the Balimo region – see Warner, Currie et al 2018 : Melioidosis in Papua New Guinea and Oceania- an important short read.

Melioidosis is caused by Burkholderia pseudomallei,  a Gram-negative rod, straight or slightly curved, with bipolar staining, or safety pin appearance. It is highly motile when viewed under phase contrast microscopy. Continue reading

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Virology overview

Consider specific diagnostic approaches to each agent, including where relevant, serological testing, antigen detection and nucleic acid amplification methods.  Also know what is locally in use in your laboratory.

Immunisation – need to know about the vaccines that are available – relevant for HPV, HBV, Influenza, Dengue, JEV, Polio, HAV, HEV, MMR, Rotavirus.

Particular attention required for those agents underlined. This is not an exhaustive listing but includes all viruses of current clinical relevance.

Reference

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